STUDY ON GENETIC VARIATION OF KELEMBAK (Rheum officinale Baill.) USING RAPD MOLECULAR MARKERS IN CENTRAL JAVA, INDONESIA
PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY,
Page 68-74
DOI:
10.56557/pcbmb/2022/v23i37-387911
Abstract
Kelembak (Rheum officinale Baill.) is native to mainland Central China and is reported as an endangered medicinal plant. This plant in Indonesia is distributed in mountainous areas; the center of rhubarb cultivation is in the Dieng Plateau. Mainly, this plant is grown for the roots as herbal medicine material (kelembak jamu) and in the industry for cigarette ingredients (kelembak Jawa). This study aimed to assess the genetic diversity of nine accessions of R. officinale. Six selected RAPD primers were used to amplify the samples and produced 33 DNA fragments ranging from 270-2,710 bp. The genetic similarity ranged from 74.58% to 100% using the Dice similarity index. The dendrogram divided R. officinale into two main clusters separated between kelembak jamu and kelembak jawa. Cluster analysis revealed that Mangli accession (kelembak jamu) had a closer similarity to kelembak Jawa. The narrow genetic diversity of kelembak is essential information for future conservation and management programs in the cultivation of this species.
Keywords:
- Genetic diversity
- kelembak
- RAPD
- Rheum officinale
- rhubarb
How to Cite
References
Barney DL, Hummer KE. Rhubarb: Botany, horticulture, and genetic resources, in Horticultural Reviews, First Edit, J. Janick, Ed. (John Wiley & Sons, Inc.). 2012;40:147–182.
Sun M, Li L, Wang M, Van Wijk E, He M, Van Wijk R, Koval S, Hankemeier T, Van Der Greef J, Wei S. Effects of growth altitude on chemical constituents and delayed luminescence properties in medicinal rhubarb. J. Photochem. Photobiol. B Biol. 2016;162:24–33.
Wang X, Feng L, Zhou T, Ruhsam M, Huang L, Hou X, Sun X, Fan K, Huang M, Zhou Y, Song J. Genetic and chemical differentiation characterizes top-geoherb and non-top-geoherb areas in the TCM herb rhubarb. Sci. Rep. 2018;8: 1–14.
Jang HW, Hsu W-H, Hengel MJ, Shibamoto T. Antioxidant activity of rhubarb (Rheum rhabarbarum L.) extract and its main component emodin. Nat. Prod. Chem. Res. 2018;06,03:4–7.
Tabin S, Kamili AN, Ganie SA, Zargar O, Sharma V, Gupta RC. Genetic diversity and population structure of rheum species in Kashmir Himalaya based on ISSR markers Flora Morphol. Distrib. Funct. Ecol. Plants, 2016;223:121–128.
Kumar J, Agrawal V. Assessment of genetic diversity, population structure and sex identification in dioecious crop, Trichosanthes dioica employing ISSR, SCoT and SRAP markers. Heliyon. 2019;5(3):e01346.
Sunar S, Korkmaz M, Siğmaz B, Ağar G. Determination of the genetic relationships among salvia species by rapd and ISSR analyses. Turkish J. Pharm. Sci. 2020;17(5):480–485.
Subositi D, Widodo H, Mujahid R, Rahmawati N, Mustofa FI, Haryanti S, Sholikhah IYM, Maruzy A, Widiyastuti Y. Genetic Diversity and Chemicals Profile of Ginger (Zingiber officinale Roscoe) in Indonesia. Int. J. Adv. Sci. Eng. Inf. Technol. 2022;12(3):929–936.
Hu Y, Wang L, Xie X, Zhang H, Yang J, Li Y. Genetic variation in cultivated rhubarb (Rheum tanguticum Maxim. ex Balf.) and the relationship with their wild relatives in China revealed by ISSR markers. Plant Syst. Evol. 2014;300(10):2217–2227.
Kuhl JC, Deboer VL. Genetic diversity of rhubarb cultivars. J. Am. Hortic. Soc. 2008;133(4):587–592.
Wang XM, Hou XQ, Zhang YQ, Yang R, Feng SF, Li Y, Ren Y. Genetic diversity of the endemic and medicinally important plant Rheum officinale as revealed by inter-simple sequence repeat (ISSR) markers. Int. J. Mol. Sci. 2012;13(3):3900–3915.
Wang X, Yang R, Feng S, Hou X, Zhang Y, Li Y, Ren Y. Genetic variation in Rheum palmatum and Rheum tanguticum (Polygonaceae), two medicinally and endemic species in China using ISSR markers. PLoS One. 2012; 7(12).
Lorenzoni RM, Menine F, Marques Júnior E, Oliveira FL, Soares TCB, Genetic diversity of yacon accessions using ISSR markers. Genet. Mol. Res. 2017;16(2): 1–8.
Suyal R, Jugran AK, Rawal RS, Bhatt ID. Morphological, phytochemical and genetic diversity of threatened Polygonatum verticillatum (L.) All. populations of different altitudes and habitat types in Himalayan region. Physiol. Mol. Biol. Plants. 2021;27(8): 1795–1809.
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