THE REPELLENT POTENTIALITY OF Origanum glandulosum (Desf.), Piper nigrum L. ESSENTIAL OILS AND THEIR COMBINATION AGAINST Tribolium confusum (Duval.)

Main Article Content

LAMAMRA MEBARKA
LAOUER HOCINE

Abstract

The use of insecticides is one of the effective methods of control of insects in stored foodstuffs, but the presence of residues in the foodstuffs and the emergence of strains of insects resistant to these insecticides are becoming more and more worrying. All these reasons militate in favor of the search for alternative methods, in particular, the use of essential oils with insecticide and/or insect repellent properties. For this purpose, the repellent effect of the essential oils of the aerial part of Origanum glandulosum (Desf.) (Lamiaceae), the seeds of Piper nigrum L. (Piperaceae) and the combination between the two oils (1/2:1/2) against the insect Tribolium confusum (Duval) were evaluated using the preferential area method on filter paper. The essential oils were obtained by hydrodistillation using a Clevenger-type apparatus, then, four different contents of these oils were prepared by dilution in acetone, in the proportions of 2, 4, 8, 12 µl/ml of acetone which corresponds to 0.031, 0.062, 0.125, 0.188 µl/cm2 respectively. The result reveals that the highest dose of O. glandulosum (Desf.) and the combination of the (O. glandulosum (Desf.) and P. nigrum L.) has a strong repellent effect of 93.33% on adults of T. confusum (Duval). Also, the combination of the two essential oils has shown the lowest RD50 (0.0013μl/cm2) followed by the oil of O. glandulosum (0.013μl/cm2). However, the oil of P. nigrum records the highest RD50 (0.025 μl/cm2). From these results, it was concluded that the repulsion percentage of the oils is dose-dependent and the RD50 varies according to the sensitivity of the insect to essential oils.

Keywords:
Origanum glandulosum (Desf.), Piper nigrum L, essential oil, Tribolium confusum (Duval), repellent activity

Article Details

How to Cite
MEBARKA, L., & HOCINE, L. (2022). THE REPELLENT POTENTIALITY OF Origanum glandulosum (Desf.), Piper nigrum L. ESSENTIAL OILS AND THEIR COMBINATION AGAINST Tribolium confusum (Duval.). PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 23(31-32), 12-18. Retrieved from https://ikpresse.com/index.php/PCBMB/article/view/7786
Section
Original Research Article

References

Smith EH, Whitman RC. Field guide to structural pests. National Pest Management Associated, Dunn Loring, VA; 1992.

Tripathi AK, Prajapati V, Aggarwal KK, Kumar S. Toxicity, feeding deterrence, and effect of activity of 1, 8-cineole from Artemisia annua on progeny production of Tribolium castanaeum (Coleoptera: Tenebrionidae). Journal of Economic Entomology. 2001;94(4):979-983.

Walter VE. Stored product pests. In Handbook of Pest Control (Story K, Moreland D. (editors). Franzak & FosterCo, Clevel and, OH. 1990;526-529.

Boussaada O, Kamel MBH, Ammar S, Haouas D, Mighri Z, Helal AN. Insecticidal activity of some Asteraceae plant extracts against Tribolium confusum. Bulletin of Insectology. 2008;61(2):283-289.

Nowaczyk K, Obrepalska-Steplowska A, Gawlak M, Throne JE, Olejarski P, Nawrot J. Molecular techniques for detection of Tribolium confusum infestations in stored products. Journal of Economic Entomology. 2009;102(4):1691-1695.

Bounechada M, Arab R. Effect insecticide des plantes Melia azedarach L. et Peganum harmala L. sur Tribolium castaneum Herbst (Coleoptera: Tenebrionidae); 2011.

Nabti LZ, Sahli F, Laouar H, Olowo-okere A, Wandjou JGN, Maggi F. Chemical composition and antibacterial activity of essential oils from the Algerian endemic Origanum glandulosum Desf. against multidrug-resistant uropathogenic E. coli isolates. Antibiotics. 2020;9(1):29.

Naima S, Fatiha B, Chahrazed B. Analytic study of the essential oil of Origanum glandulosum (Desf.) from Algeria. Journal of Essential Oil-Bearing Plants. 2007;10(2):145-150.

Ashokkumar K, Murugan M, Dhanya MK, Pandian A, Warkentin TD. Phytochemistry and therapeutic potential of black pepper [Piper nigrum (L.)] essential oil and piperine: A review. Clinical Phytoscience. 2021;7(1):1-11.

Morshed S, Hossain MD, Ahmad M, Junayed M. Physicochemical characteristics of essential oil of black pepper (Piper nigrum) cultivated in Chittagong, Bangladesh. Journal of Food Quality and Hazards Control. 2017; 4(3):66-69.

Dosoky NS, Satyal P, Barata LM, daSilva JKR, Setzer WN. Volatiles of black pepper fruits (Piper nigrum L.). Molecules. 2019;24(23):4244.

McDonald LL, Guyr H, Speire RR. Preliminary evaluation of new candidate materials as toxicants, repellents and attracts against stored product insect. Marketing Research. 1970;189.

Arab R, Lemeailbi N, Benhissen S. Repellent activity of essential oils from Artemisia Herba Alba Asso. and Teucrium polium L. against two stored product insects. Nveo-Natural Volatiles & Essential Oils Journal|NVEO. 1508-1515.

Finney DJ. Probit analysis. 3rd ed Cambridge Univ. Press. London, UK; 1971.

Khalfi O, Sahraoui N, Bentahar F, Boutekedjiret C. Chemical composition and insecticidal properties of Origanum glandulosum (Desf.) essential oil from Algeria. Journal of the Science of Food and Agriculture. 2008;88(9):1562-1566.

Bouchikhi-Tani Z, Khelil MA, Bendahou M. Evaluation des propriétés larvicides des huiles essentielles extraites de cinq plantes aromatiques d’Algérie : essai sur la mite Tineola bisselliella (Lepidoptera : Tineidae). Lebanese Science Journal. 2018 ;19(2) :187- 199.

Upadhyay RK, Jaiswal G. Evaluation of biological activities of Piper nigrum oil against Tribolium castaneum. Bulletin of Insectology. 2007;60(1):57.

Vinturelle R, Mattos C, Meloni J, Nogueira J, Nunes MJ, Vaz IS, Chagas EFD. In vitro evaluation of essential oils derived from Piper nigrum (Piperaceae) and Citrus limonum (Rutaceae) against the tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). Bio Chemistry Research International; 2017.

Ishii T, Matsuzawa H, Vairappan CS. Repellent activity of common spicesagainst the rice weevil, Sitophilus zeamais Motsch (Coleoptera, Curcu lionidae). Journal of Tropical Biology & Conservation (JTBC); 2010.

Tchoumbougnang FO, Jazet DPM, Sameza ML, Fombotioh N, Vyry WNA, Henri AZP, Menut C. Comparative essential oils composition and insecticidal effect of different tissues of Piper capense L, Piper guineense Schum. et Thonn, Piper nigrum L. and Piper umbellatum L. grown in Cameroon. African Journal of Biotechnology. 2009;8(3).