INDUCTION OF SOMATIC EMBRYOS FROM Ruta graveolens. L: AN IMPORTANT MEDICINAL AND AROMATIC PLANT

Main Article Content

AJIT KENGAR
https://orcid.org/0000-0002-9054-4540

Abstract

Somatic embryogenesis is process by which plant vegetative cell undergoes restructuring to generate embryogenetic cells.These embryogenic potential cells then go through a series of morphological and biochemical changes that result in the formation of somatic or non-zygotic embryo capable of regenerating plants.Somatic embryogenesis provides a valuable tool in genetic improvement of commercial crop species.The present study was undertaken to induce somatic embryos from Ruta graveolens by using plant growth regulators(PGR).In the present investigation evaluation of the most suitable concentration of plant growth regulators for induction in Ruta graveolens is made. Callus was initiated from inter nodal portion of stem,on Murashige-Skoog’s (MS) basal media supplemented with various combinations of auxins and cytokinines.The callus obtained from intermodal segment explants was cultured on MS medium supplemented with various combinations of PGR’s.

Studies were also  carried out to evaluate the induction of somatic embryo regeneration capacity of callus using plant growth regulators on growth of Ruta graveolens L.Among the different concentration of plant growth regulators used in the study;it was found that MS fortified with 4.44 µM BAP+2.69µM NAA found suitable for induction of somatic embryo.This study would help for large-scale production of R. graveolens.

Keywords:
: Somatic embryogenesis, Ruta graveolens, plant growth regulators, callus

Article Details

How to Cite
KENGAR, A. (2021). INDUCTION OF SOMATIC EMBRYOS FROM Ruta graveolens. L: AN IMPORTANT MEDICINAL AND AROMATIC PLANT. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 22(37-38), 8-15. Retrieved from https://ikpresse.com/index.php/PCBMB/article/view/6349
Section
Short Research Article

References

Bradly PR. British herbal compendium. Bournemouth: British Herbal medicine Association. 1998;1.

Berdonces JL. Gran enciclopedia de las plantas medicinales. Barcelona: Tikal; 1998.

De Feo V, Simonea De F, Senatoreb F. Potential allelochemicals from the essential oil of Ruta graveolens. Phytochemistry. 2002;61:573–578.

Kleijnen J, Knipschild P, Ter Riet G. Clinical trials of homoeopathy. British Medical Journal, February. 1991;9(302): 316-323.

Foucaud A. Contributions a l'etude des plantes medicinales du Nord-Vietnam. Trav Laboratory Materia Medica Pharmacia galnica (Faculty of Pharmacy, Paris). 1953;38(1):1 - 206.

Tomar UK. Gupta SC. Somatic embryogenesis and organogenesis in callus cultures of a tree legume—Albizia richardiana King. Plant Cell Reports. 1988;7(1):70-73.

Castro RL, Barros IBI. Micropropagaton of rue (Ruta graveolens). Acta Horticulturae. 1999;50(2):325 – 328.

Faisal M, Ahmad N, Anis M. In Vitro regeneration and mass propagation of Ruta graveolens L. A multipurpose shrub. Horticulture Science. 2005;40:1478-1480.

Bohidar SM, Thirunavoukkarasu, Rao TV. Effect of plant growth regulators on in vitro micropropagation of ‘Garden rue’ (Ruta graveolens L.). International Journal of Integrative Biology. 2008;3(1):36 - 43.

Tejavathi DH, Manjula BL. Studies on organogenesis from nodal explant of Ruta .graveolens L. The Bioscan. 2010;5(3): 455-459.

Ajit Kengar, Govind Paratkar. In vitro micropropagation of ‘Garden Rue’ (Ruta graveolens L.) by Direct organogenesis International Journal of Advanced Life Sciences. 2015;8(2):178-183

Zuraida AR, Mohd Shukri MA, Erny Sabrina MN, Fatin Liyana Izzati K, Ayu Nazreena O, Juhazliana, Wan Zaliha WS. Improved plant regeneration and in vitro somatic embryogenesis in Ruta graveolens Journal of Experimental Biology and Agricultural Sciences. June - 2014;2(3): 328-336.

Stasolla C, Yeung EC. Recent advances in conifer somatic embryogenesis: improving somatic embryo quality. Plant Cell, Tissue and Organ Culture. 2003;74:15–35.

Murashige T, Skoog F. A revised medium for rapid groth and bioassays with tobacco tissue cultures. Physiologia Plantarum. 1962;15:473-497.

Kengar Ajit, Paratkar Govind. Effect of plant growth regulators on In –Vitro callogenesis In Ruta graveolens. L. Bionano Frontier. 2015;7(1):Jan 4-8.

Geovanny I Nic-Can, Víctor M Loyola-Vargas. The role of the auxins during somatic embryogenesis Springer International Publishing: Cham, Switzerland. 2016;415–43.

Shabana MM, El-Alfy TS. El-Tantawy ME, Ibrahim AI, Ibrahim GF. Tissue culture and evaluation of some active constituents of Ruta graveolens L. II: Effect of plant growth regulators, explant type and precursor on coumarin content of Ruta graveolens L. callus cultures. Arab J. Biotech. 2002;5(1) Jan:45-56.

Gurudeeban S, Satyavani, K, Ramanathan T, Balasubramanian T. Effect of antioxidant and anti-aggregating properties of micro-propagated plantlets of Ruta graveolens L. Afr. J. Biotechnol. 2011;11(6):1497-1504.

Srivastava P, Pandey A. Standardization of callus induction and plant regeneration from leaf explants of Black Gram (Vigna mungo var. silvestris). International Journal of Innovations in Biological and Chemical Sciences. 2011;1:1-6.

Bhaskaran S, Smith RH. Regeneration in cereal tissue culture: A review. Journal of Crop Science. 1990;30:1328-1336.

Magali F. Grando1, Chandra I. Franklin Robert G. Shatters Jr. Optimizing embryogenic callus production and plant regeneration from ‘Tifton 9’ bahiagrass seed explants for genetic manipulation, Plant Cell, Tissue and Organ Culture. 2002; 71:213–222.

Ramakrishna D, Shasthree T. High efficient somatic embryogenesis development from leaf cultures of Citrullus colocynthis (L.) Schrad for generating true type clones. Physiol Mol Biol Plants. Apr; 2016;22(2):279–285.

Timir baran Jha, Priyanka Mukherjee, Mukul Manjari Datta. Somatic embryogenesis in Jatropha curcas Linn., an important biofuel plant. Plant Biotechnology Reports. 2007;1:135– 140.