MYCOSYNTHESIZE OF AG-NANOPARTICLES BY Penicillium expansum AND ITS ANTIBACTERIAL ACTIVITY AGAINST BACTERIAL PATHOGENS

Main Article Content

RAGHDA S. ISMAIL
REYAD M. EL-SHARKAWY
BASMA H. AMIN
MAHMOUD A. SWELIM

Abstract

Multidrug-resistant pathogens have promoted research of nontraditional antibiotic agents. Several classes of nano-antibiotics have affirmed their effectiveness against various eukaryotic microorganisms including bacteria, viruses and fungi. The production of silver nanoparticles using Penicillium expansum was investigated. Ag-nanoparticles were characterized by UV–visible spectrum, energy dispersive X-ray, zeta sizer Nano S90 and transmission electron microscopy. Biosynthesize Ag+ nanoparticles were confirmed by 420 nm corresponding to a surface plasmon resonance. EDX has confirmed the presence of the silver element. TEM micrographs showed irregular, spherical, hexagonal and monodispersed nanoparticles of 9–18 nm. Bio-Ag nanoparticles showed a significant antibacterial effect on Methicillin-Resistant Staphylococcus aureus RCMB 010010. The results showed a cytotoxicity activity on the human breast cancer (MCF-7) cell line in a concentration-dependent manner. Thus, biosynthesized Ag-nanoparticles are eco-friendly antibacterial and antitumor compounds.

Keywords:
Silver nanoparticles, antibacterial activity, ultrastructure, cytotoxicity.

Article Details

How to Cite
ISMAIL, R. S., EL-SHARKAWY, R. M., AMIN, B. H., & SWELIM, M. A. (2021). MYCOSYNTHESIZE OF AG-NANOPARTICLES BY Penicillium expansum AND ITS ANTIBACTERIAL ACTIVITY AGAINST BACTERIAL PATHOGENS. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 22(9-10), 13-25. Retrieved from https://ikpresse.com/index.php/PCBMB/article/view/5950
Section
Original Research Article

References

Amin BH, El-Sharkawy RM. Bactericidal activity of silver nanoparticles produced by Fusarium solani against the Multidrug- Resistant Bacteria. Res. J. Pharma. Biol. Chem. Sci. 2019;10(6):203–211.

Singh D, Vandana R, Shivaraj N, Jyothi H, Singh K, Mathew J. Optimization and characterization of silver nanoparticle by endophytic Fungi penicillium sp. Isolated from Curcuma longa (Turmeric) and Application Studies against MDR E. coli and S. aureus. Bioinorg. Ch. Appl. Article ID 408021. 2014;8.

Ouay B, Stellacci F. Antibacterial activity of silver nanoparticles: a surface science insight. Nano. Today. 2015;10:339–354.

Patra JK, Baek KH. Antibacterial activity and synergistic antibacterial potential of biosynthesized silver nanoparticles against foodborne pathogenic bacteria along with its anticandidal and antioxidant effects. Front. Microbiol. 2017;8:167.

Mohanpuria P, Rana N, Yadav S. Biosynthesis of nanoparticles: Technological concepts and future applications. J. Nanoparticle. Res. 2008; 10(3):507–517.

Verma VC, Kharwar RN, Gange AC. Biosynthesis of antimicrobial silver nanoparticles by the endophytic fungus Aspergillus clavatus. Nanomed. 2010;5:33–40.

Kashyap PL, Kumar S, Srivastava AK, Sharma AK. Biosynthesis of antimicrobial silver nanoparticles by the endophytic fungus Aspergillus clavatus. W. J. Microbiol. Biotechnol. 2013;29:191–207.

Musarrat J, Dwivedi S, Singh BR, Al-Khedhairy AA, Azam A, Naqvi A. Production of antimicrobial silver nanoparticles in water extracts of the fungus Amylomyces rouxii strain KSU-09. Biores. Technol. 2010;101:8772–8776.

Singh R, Shedbalkar U, Wadhwani A, Chopade A. Bacteriagenic silver nanoparticles: synthesis, mechanism, and applications. Appl. Microbiol. Biotechnol. 2015;99:4579–4593.

Hulkoti NI, Taranath TC. Biosynthesis of nanoparticles using microbes—A review. Coll. Surf. B. Biointerf. 2014;121: 474–483.

Shaligram NS, Bule M, Bhambure R, Singhal SR, Singh SK, Zakacs G, Pandey A. Biosynthesis of silver nanoparticles using aqueous extract from the compactin producing fungal strain. Pro. Biochem. 2009;44:939–943.

Ferdous Z, Nemmar A. Health impact of silver nanoparticles: A review of the biodistribution and toxicity following various routes of exposure. Int J Mol Sci. 2020;21(7):2375.
DOI: 10.3390/ijms21072375

Mazur P, Skiba-Kurek IA, Mrowiec P, Karczewska E, Drożdż R. Synergistic ROS-Associated antimicrobial activity of silver nanoparticles and gentamicin against Staphylococcus epidermidis Int J Nanomedicine. 2020;19;15:3551-3562.
DOI: 10.2147/IJN.S246484

Rodriguez PL, Harada T, Christian DA, Patano DA, Tsai RK, Discher DE. Minimal ‘self’ peptides that inhibit phagocytic clearance and enhance delivery of nanoparticles. Sci. 2013;339:971.

Hamedi S, Shojaosadati S, Shokrollahzadeh S, Hashemi-Najafabadi S. Extracellular biosynthesis of silver nanoparticles using a novel and non-pathogenic fungus, Neurospora intermedia: Controlled synthesis and antibacterial activity. W. J. Micro. Bio. 2014;30:693–704.

Sarkar S, Atish D, Samir K, Golam M. Extracellular biosynthesis of silver nanoparticles using a novel and non-pathogenic fungus, Neurospora intermedia: Controlled synthesis and antibacterial activity. Poly. 2007;26:4419–4426.

Amin BH, Abou-Dobara MI, Diab MA, Gomaa EA, El-Mogazy MA, El-Sonbati AZ, EL-Ghareib MS, Hussien MA, Salama HM. Synthesis, characterization, and biological investigation of new mixed-ligand complexes. Appl. Organomet. Chem. 2020;1-18.

Selvi C, Madhavan J, Santhanam A. Cytotoxic effect of silver nanoparticles synthesized from Padina tetrastromatica on breast cancer cell line. Adv. Nat. Sci. Nanosci. Nanotechnol. 2016;7: 035015(8).

Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol. Methods. 1983;65:55-63.

Vijayan P, Raghu C, Ashok G, Dhanaraj SA, Suresh B. Antiviral activiy of medicinal plants of Nilgiris. Indian. J. Med. Res. 2004;120:24-29.

Amin BH, Ahmed HY, Abd El- Aziz MM. Bactericidal activity of Silver Nanoparticles produced by Fusarium Solani against the Multidrug- Resistant Bacteria. N. E. J. M. 2018;50:80-97.

Ghoneimy EA, El Shikh HH, Elaasser MM, Ahmed HYH. The cytotoxic effects of certain fungal filtrates against three tumor cell lines. Afr. J. Mycol. Biotech. 2012; 17(3):21-38.

Tran QH, Le A. Silver nanoparticles: Synthesis, properties, toxicology, application and perspectives. Adv. Nat. Sci. Nanosci. Nanotech. 2013;4(3):033001.

Gopinath V, Mubarak A, Priyadarshini S, Priyadharsshini NM, Thajuddin N, Velusamy P. Biosynthesis of silver nanoparticles from Tribulus terrestris and its antimicrobial activity: A novel biological approach. Coll. Surf .B. Bioint. 2012;96:69–74.

Shukla MK, Singh RP, Reddy CRK, Jha B. Synthesis and characterization of agar-based silver nanoparticles and nanocomposite film with antibacterial applications. Bioresour. Technol. 2012; 107:295–300.

Barkhade T. Extracellular biosynthesis of silver nanoparticles using Sspecies. Int. J. Res. – Granthaalayah. 2018;6(1):277–283.

Bhainsa KC, D′souza S. Extracellular biosynthesis of silver nanoparticles using the fungus Aspergillus fumigatus. Coll. Surf. B. Biointer. 2006;47(2):160–164.

Sadhasivam S, Shanmugam P, Yun K. Biosynthesis of silver nanoparticles by Streptomyces hygroscopicus and antimicrobial activity against medically important pathogenic microorganisms. Coll. Surf. B. Biointerf. 2010;81:358–362.

Gengan RM, Anand K, Phulukdaree A, Chuturgoon A. A549 lung cell line activity of biosynthesized silver nanoparticles using Albizia adianthifolia leaf. Coll. Surf. B. Biointerf. 2013;105:87–91.

Aiad I, El-Sukkary M, Soliman EA, El-Awady MY, Shaban SM. Evaluation of some cationic surfactants based on dimethylaminopropylamine as corrosion inhibitors. J. Ind. Eng. Chem. 2014;21: 1029-1038.

Ghosh S, Patil S, Ahire M, Kitture R, Kale S, Pardesi K, Cameotra S, Belleare J, Dhavale D, Chopade BA.. Synthesis of silver nanoparticles using Dioscorea bulbifera tuber extract and evaluation of its synergistic potential in combination with antimicrobial agents. Int. J. Nanomed. 2012;7:483–496.

Ammar HA, El-Desouky TA. Green synthesis of nanosilver particles by Aspergillus terreus HA1N and Penicillium expansum HA2N and its antifungal activity against mycotoxigenic fungi. J. Appl. Microbiol. 2016;121:89-100.

Hu CMJ, Zhang L, Aryal S, Cheung C, Fang RH, Zhang L. Erythrocyte membrane-camouflaged polymeric nanoparticles as a biomimetic delivery platform. P. N. A. S. 2011;108:10980–10985.

Husseiny S, Taher AS, Hend AA. Biosynthesis of size controlled silver nanoparticles by Fusarium oxysporum, their antibacterial and antitumor activities. J. Basic. Appl. Sci. 2015;4:225–231.

Singh T, Kumari J, Patnaik A, Singh A, Chauhan R, Chande S. Biosynthesis, characterization and antibacterial activity of silver nanoparticles using an endophytic fungal supernatant of Raphanus sativus. J. Gen. Eng. Biotechn. 2017;15: 31–39.

Sriram MI, Kanth M, Kalishwaralal K, Gurunathan S. Antitumor activity of silver nanoparticles in Dalton's lymphoma ascites tumor model. Int. J. Nanomed. 2010;5:753–762.

Suganya G, Karthi S, Shivakuma MS. Larvicidal potential of silver nanoparticles synthesized from Leucas aspera leaf extracts against dengue vector Aedes aegypti. Para. Res. 2014;113:875–880.

Majeed S, Mohd S, Anima N, Mohammed T. In vitro study of the antibacterial and anticancer activities of silvernanoparticles synthesized from Penicillium brevicompactum (MTCC-1999). J. Tai. Uni. Y. Sci. 2016;10:614–620.

Wypij M, Jędrzejewski T, MACIEJ OI, Trzcińska J, Rai M, Golińska1 P. biogenic silver nanoparticles: assessment of their cytotoxicity, genotoxicity and study of capping proteinsmolecules. 2020;25(13): 3022.
DOI: 10.3390/molecules25133022

Ullah I, Talha A, Ali M, Iqbal J, Ali W, Alarifi S, Khan Z. Green-synthesized silver nanoparticles induced apoptotic cell death in MCF-7 breast cancer cells by generating reactive oxygen species and activating caspase 3 and 9 enzyme activities. Oxidative medicine and cellular longevity; 2020.
Available:https://doi.org/10.1155/2020/1215395