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The role of fungi based nutraceuticals in the treatment and management of Sickle cell has been under exploited and thus necessitates for more research. This research, studies the potential of decoction extract of Trametes hirsuta mushroom in lysis reduction and stabilization of erythrocyte membrane integrity. Freshly harvested, washed and air-dried T. Hirsuta was ground and 1.5 dm3 of distilled water was added to 1.5 kg of the ground mushroom. The filtrate obtained after boiling for 30 min. and cooling for 2 hrs was dried using an autodesicator to give decoction extract. Volumes of 5.0 mg/mL, 10.0 mg/mL and 15.0 mg/mL of the extract were obtained via serial dilutions, while HbS erythrocytes were obtained by centrifuging 5.0 mL of HbS-blood mixed in 5.0 mL of 0.9 % physiological saline. HbS erythrocytes treated with 15 mg/mL and 10 mg/mL had the highest and the lowest lysis at 50% mark. Lowest median corpuscular fragility was observed in erythrocytes suspended in 10.0 mg/mL of extract, while negative percentage stability were observed in erythrocytes suspended in 10.0 mg/mL and 5.0 mg/mL of extract. The control had the highest hemoglobin polymerization, while morphological stability was highest (89.40 + 4.61 %) after 5 min. of culturing in 10.0 mg/mL of extract. The ability of this mushroom extract to stabilize erythrocyte membrane integrity and reduce the progression of lysis shows that this mushroom can be exploited as a possible nutraceutical means in both the management and treatment of sickle cell anemia and its associated crises.
Barceloux DG. Selenium. Clin. Toxicol. 1999;37(2):145-172.
Hobbs CJ. Medicinal mushrooms: An exploration of tradition, healing and culture. Portland, Oregon: Culinary Arts Ltd. Oregon. 1995;20 - 21.
Sullivan R, Smith JE, Rowan NJ. Medicinal mushrooms and cancer therapy: Translating a traditional practice into Western medicine. Perspect. Biol. Med. 2006;49(2):159-170.
Borchers AT, Krishnamurthy A, Keen CL, Meyers FJ, Gershwin ME. The Immunobiology of Mushrooms. Exp. Biol. Med. 2008;233(3):259 - 276.
Falandysz J. Selenium in edible mushrooms. Jour. Environ. Sci. Health, Part C. 2008;26: 56 - 299.
Phillips R. Mushrooms. Macmillan Publishing Co., New York. 2006;317 - 318.
Ma R, Yang R, Liu X, Chen Z, Yang C, Wang S. Chemical composition and immunomodulatory activity of mycelia of the hairy bracket mushroom, Trametes hirsuta (Higher Basidiomycetes). Inter. Jour. Med. Mushrooms. 2015;17:267 - 276.
Dacie JV, Lewis SM, Luzzatto L. Investigation of the hereditary haemolytic anaemia membrane and enzyme abnormalities in practical haematology, Churchill Livingstone, New York. 1981;234 – 238.
Dewey MJ, Brown JL, Nallaseth FS. Genetic differences in red cell osmotic fragility: analysis in allophonic mice. Blood. 1982;59(5):986 - 989.
Krogmeier DE, Mao IL, Bergen WG. Genetic and nongenetic effects of erythrocyte osmotic fragility in lactating Holstein Cows and its association with yield traits. Jour. Diary Sci.; 1994; 2000 -2001.
Chikezie PC, Chikezie CM, Nwanegwo CO. Osmotic fragility index of chicken (Gallus gallus) and human HbAA erythrocytes. Inter. Sci. Res. Jour. 2008;1(1):1 - 4.
Ohiri RC, Essien EB. Stabilizing Ability and Anti-sickling Potentials of Ganoderma Lucidum Decoction Extract on Human HbS Erythrocytes Membrane. Am. Jour. Biomed. Sci. 2018;10(4):202 - 210.
Noguchi, C. T. and Schetcher, A. N. The inhibition of sickle cell hemoglobin by amino acids and related compounds. Biochem. 1978;17:5455 - 5459.
Iwu MN, Igboko OO, Onwubiko H, Ndu UP. Effect of Cajanus cajan on gelation and oxygen affinity of sickle cell haemoglobin. Jour. Ethnopharm. 1988;22:99 - 104.
Daland GA, Castle WB. A simple and rapid method for demonstrating sickling of the red blood cells. The use of reducing agents. Jour. Lab. Clin. Med. 1984;33:1085.
Brown BA. Hematology: Principles and procedures, 5th ed., Lippincott Williams and Wilkins, Philadelphia. 1993;96 - 97.
Parpart AK, Lorenz PB, Parpart ER., Gregg JR, Chase AM. The osmotic resistance (fragility) of human red cells. Jour. Clin. Investig. 1947;26:636 - 638.
Tamer L, Polat G, Yücebilgiç G, Güvenç B, Başlamışlı F. The levels of sera malondialdehyde, erythrocyte membrane Na+-K+/Mg++ and Ca++/Mg++ adenosine 5’ triphosphatase in patients with sickle cell anemia. Tur. Jour. Haemat. 2000;17:23 - 26.
Rotuck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG. Selenium: Biochemical role as a component of glutathione peroxidase. Sci.; 1973;179:588 - 590.
Burk RF. Selenium. In: Olson. R. E., Broquist, H. P., Chichester, C. O., Darby, W. J., Kolbye Jr, A. C., and Slavey, R. M. editors. Present knowledge in nutrition, 5th ed. Nutrition Foundation Inc., Washington D. C. 1984;519 - 527.
Chikezie PC. Sodium metabisulfite-induced polymerization of sickle cell hemoglobin incubated in the extracts of three medicinal plants (Anacardium occidentale, Psidium guajava, and Terminalia catappa). Pharmacog. Mag. 2011;7:126 - 132.
Ohiri RC, Odey OP. Human hemoglobin s erythrocyte-stabilizing and antisickling potential of extract of wood ear mushroom, Auricularia auricula (Agaricomycetes). International Journal of Medicinal Mushrooms. 2022;24(3).