ANTIMICROBIAL SCREENING OF THE LEAF EXTRACTS OF Cissus aralioides (Welw. ex Baker) Planch. AND Cissus polyantha Gilg & Brandt.

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Plants are valuable sources of natural products with great potentials for producing new drugs. This study aimed at evaluating the antimicrobial activities of acetone, ethanol and aqueous leaf extracts of Cissus aralioides and Cissus polyantha against Staphylococcus aureus, Escherichia coli, Psuedomonas aeruginos, Salmonella typhi and Candida albicans using the agar well diffusion method. The zones of inhibition were measured in millimeters (mm) and presented as mean values with the standard error. The ethanol extract of C. polyantha showed the highest zone of inhibition of 12 mm against P. aureginosa at a concentration of 100 mg/ml. This was significantly lower than the activities of the standard antibiotics against the same pathogen.  Similarly, the observed zone of inhibition (6.5 mm) of acetone extracts of C. polyantha against S. typhi, was lower than the standard antibiotics with a range of 22.55\(\pm\)0.43 mm to 32.75\(\pm\) 0.82 mm. Cold aqueous extracts of both species showed no inhibition zone. The leaf extracts of the two plant species did not significantly inhibit bacterial and fungal growths which suggests that C. polyantha and C. aralioides leaf extracts may not be effective in the treatment of microbial infections caused by the test organisms used in this study.

Cissus aralioides, Cissus polyantha, antimicrobial, inhibition, aqueous extract

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AGOGBUA, J. U., & SADUWA, O. (2022). ANTIMICROBIAL SCREENING OF THE LEAF EXTRACTS OF Cissus aralioides (Welw. ex Baker) Planch. AND Cissus polyantha Gilg & Brandt. Asian Journal of Plant and Soil Sciences, 7(1), 305-312. Retrieved from
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Atanasov AG, Waltenberger B, Pferschy-Wenzig EM, Linder T, Wawrosch C, Uhrin P, Temml V, Wang L, Schwaiger S, Heiss EH, Rollinger JM. Discovery and resupply of pharmacologically active plant-derived natural products: A review. Biotechnology Advances. 2015;33(8):1582-614.

Jamshidi-Kia F, Lorigooini Z, Amini-Khoei H. Medicinal plants: Past history and future perspective. Journal of Herbmed Pharmacology. 2018;7(1).

Matole V, Thorat Y, Ghurghure S, Ingle S, Birajdar A, Nangare G, Safwan M, Madur S, Patil S, Bagalkote Z, Sakhare A. A brief review on Herbal Medicines. Research Journal of Pharmacognosy and Phytochemistry. 2021; 13(2):101-2.

Zangeneh MM. Green synthesis and formulation a modern chemotherapeutic drug of Spinacia oleracea L. leaf aqueous extract conjugated silver nanoparticles; Chemical characterization and analysis of their cytotoxicity, antioxidant, and anti-acute myeloid leukemia properties in comparison to doxorubicin in a leukemic mouse model. Applied Organometallic Chemistry. 2020; 34(1):e5295.

Gupta J, Gupta R, Kalra V, Wahi N. Application of medicinal plants in management of endogenous bioactive molecules as potential biomarkers for cardiovascular disease and disorders. Current Trends in Biotechnology and Pharmacy. 2019;13(3):350-65.

O’neill J. Antimicrobial resistance. Tackling a crisis for the health and wealth of nations; 2014.

Mohammadi A, Nazari H, Imani S, Amrollahi H. Antifungal activities and chemical composition of some medicinal plants. Journal de Mycologie Medicale. 2014;24(2):e1-8.

Singh G, Kumari B, Sinam G, Kumar N, Mallick S. Fluoride distribution and contamination in the water, soil and plants continuum and its remedial technologies, an Indian perspective–a review. Environmental Pollution. 2018;239:95-108.

Pang M, Yao Z, Chen C, Lei X, Cheng B. Human-microorganism mutualism theory: Possible mechanisms for the delayed chronic wound healing process. Medical Hypotheses. 2020;141:109720.

Von Wintersdorff CJ, Penders J, Van Niekerk JM, Mills ND, Majumder S, Van Alphen LB, Savelkoul PH, Wolffs PF. Dissemination of antimicrobial resistance in microbial ecosystems through horizontal gene transfer. Frontiers in Microbiology. 2016;173.

Reygaert WC. An overview of the antimicrobial resistance mechanisms of bacteria. AIMS Microbiology. 2018;4(3):482.

Iwu CD, Korsten L, Okoh AI. The incidence of antibiotic resistance within and beyond the agricultural ecosystem: A concern for public health. Microbiologyopen. 2020;9(9): e1035.

Ge-loud O. Natural products discovery and potential for new antibiotics. Current Opinion in Microbiology. 2019;51:81-7.

World Health Organization. Antimicrobial resistance: Global report on surveillance. Geneva: World Health Organization; 2014.

Van Duin D, Doi Y. The global epidemiology of carbapenemase-producing Enterobacteriaceae. Virulence. [Internet]. 2017; 8(4):460–9.

Available: PMID: 27593176.

Mahomoodally MF, Gurib-Fakim A, Subratty AH. Antimicrobial activities and phytochemical profiles of endemic medicinal plants of Mauritius. Pharmaceutical Biology. 2005;43(3):237-42.

Boakye–Yiadom K, Konning GH. Incidence of antibacterial activity in the Connaraceae. Planta Medica. 1975;28(08):397-400.

Burman S, Bhattacharya K, Mukherjee D, Chandra G. Antibacterial efficacy of leaf extracts of Combretum album Pers. against some pathogenic bacteria. BMC Complementary and Alternative Medicine. 2018;18(1):1-8.

Amini SM. Preparation of antimicrobial metallic nanoparticles with bioactive compounds. Materials Science and Engineering; C. 2019;103:109809.