EFFECT OF WATER STRESS ON THE GERMINATION OF BROME GRASSES (Bromusspp.) SEEDS USING POLYETHYLENE GLYCOL 6000

Main Article Content

RAFIKA LEBAZDA
MERIEM HANI
MOHAMED FENNI

Abstract

The germination phase is one of the most sensitive to the effects caused by the stress steps. The present work deals with the impact of water deficit expressed by lowering the water potential on germination percentage and the average germination time in three species of Bromus: B. madritensis L., B. lanceolatus Roth. and B. rubens L. Water stress was simulated using polyethylene glycol 6000 (PEG6000), according to different levels of water potential (- 0, 03; - 0, 7; - 1; -1,6 M Pa). The results obtained showed that water stress has a negative effect on the germination potential of the three species; the germinative behavior of these species under water stress, differs according to the species and the concentration of the PEG 6000 applied for most studied variables. Indeed, a maximum of 100%, 90% and 86% was achieved respectively for B. madritensis, B. rubens, B. lanceolatus to the water potential 0 MPa. At the lowest water potential (-1.6 MPa), the percentage of germination was slightly reduced, and was about 100%, 80%, 83.33%, respectively for the three species. Concerning the average germination time, there are no differences between the species studied, it lengthens slightly depending on the intensity of the water stress. In the light of these results, differences in sensitivity between the species appear and allow them to be classified according to the following decreasing tolerance sequence: B. madritensis> B. lanceolatus> B. rubens.

Keywords:
Bromussp, water stress, germination, weeds, tolerance, polyethylene glycol 6000.

Article Details

How to Cite
LEBAZDA, R., HANI, M., & FENNI, M. (2021). EFFECT OF WATER STRESS ON THE GERMINATION OF BROME GRASSES (Bromusspp.) SEEDS USING POLYETHYLENE GLYCOL 6000. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 22(13-14), 40-46. Retrieved from http://ikpresse.com/index.php/PCBMB/article/view/5999
Section
Original Research Article

References

Ditomaso JM, Kyser GB. Weed control in natural areas in the Western United States. Weed Research and Information Center (WRIC). University of California. 2013; 544.

Hani H, Lebazda R, Fenni M. Variations of weeds seeds of species belonging to poaceae on the basis of germination, production and morphological characteristics. Advances in Research. 2017;10(3):1-8.

Lebazda R, Fenni M, Hani M, Boukhebti H. A survey of weed seed production in vegetable crops of Setifian high plateau. Advances in Environmental Biology. 2016;10:131-136.

Fenni M. Study of weeds of winter cereals Constantine high plains, ecology, dynamics, phenology and biology of brome, Doc. Thesis, Biol. Inst; Setif. 2003;165.

Kumar RR, Karajol K, Naik GR. Effect of polyethylene glycol induced water stress on physiological and biochemical responses in pigeonpea (CajanuscajanL. Millsp.). Recent Research in Science and Technology. 2011;3(1):148-152.

Obroucheva NV, Sinkevich IA, Lityagina SV, Novikova GV. Water relations in germinating seeds. Russian Journal of Plant Physiology. 2017;64(4):625–633.

Rauf M, Munir M, Hassan MU, Ahmad M, Afzal M. Performance of wheat genotypes under osmotic stress at germination and early seedling growth stage. Afric. J. Biotechnol. 2007;6:971-975.

Khayatnezhad M, Gholamin R, Jamaati-e-Somarin Sh, Zabihi-e-Mahmoodabad R. Effects of Peg stress on corn cultivars (Zea mays L.) At Germination Stage. World Applied Sciences Journal. 2010;11(5):504-506.

Partheeban C, Chandrasekhar CN, Jeyakumar P, Ravikesavan R, Gnanam R. Effect of PEG induced drought stress on seed germination and seedling characters of maize (Zea mays L.) Genotypes. Int. J. Curr. Microbiol. App. Sci. 2017;6(5):1095-1104.

Mazliak P. Croissance et développement, 2 tomes, Herman, Paris, France. 1982;465.

Huseyin O, Shahid F, Hikmet G, Cumali O, Halil E. Higher tolerance to abiotic stresses and soil types may accelerate common ragweed (Ambrosia artemisiifolia) invasion. Weed Sci. 2001;65:115–127.

Mirbahar AA, Saeed R, Markhand GS. Effect of polyethylene glycol-6000 on wheat (Triticum aestivum L.) seed germination, International Journal of Biology and Biotechnology. 2013;10(3): 401-405.

Michel BE,Kaufman RM. The osmotic potential of polyethylene glycol 6000. Plant. Physiol. 1973;51:914-916.

Côme D. Les obstacles à la germination, Ed. Masson & Cie, Paris. 1970;162.

Ashraf M, Mehmood S. Response of four Brassica species to drought stress. Envir. Expt. Bot. 1990;1:93-100.

Ndour P,Danthu P. Effet des contraintes hydriques et salines sur la germination de quelques acacias africains. In: Campa C., Grignon C, Gueye M, & Hamon S., eds. Colloques et séminaires: l’acacia au Sénégal. Paris: Orstom. 1998;105-122.

Hopkins WG. Physiologie végétale traduction de la 2ème édition américaine par SERGE Rambour. Révision scientifique de Charle Marie Evrard. Edition DEBOEK Université, Bruxelles. 2003;514.

Jokanović MB, Zdravković J. Germination of tomatoes under PEG-induced drought stress. Ratar. Povrt. 2015;52(3):108-113.

Bewley JD. Seed germination and dormancy. The Plant Cell. 1997;9:1055-1066.

Jaouadi W, Hamrouni L, Souayeh N,Khouja ML.Étude de la germination des graines d ’Acacia tortilissous différentes contraintes abiotiques. Biotechnol. Argon. Soc. Environ.2010;14(4):643-652.

Wu L-M, Fang Y, Yang H-N, Bai L-Y.Effects of drought-stress on seed germination and growth physiology of quincloracresistant Echinochloacrus galli. PLoS ONE. 2019;14(4):e0214480.

Hamadache A, Makhlouf M et Harkati N. Effet de la date et de l’outil de travail de sol sur le comportement des bromes (Bromussp) et le rendement de blé dure (Triticum durum). Dans la région de Sétif, Céréaliculture. 2002;37:24-29.

Botia P, Carvajal M, Cerda A, Martinez V. Response of eight Cucumismelo cultivars to salinity during germination and early vegetative growth. Agronomie, 1998;18: 503-513.

Gill PK, Sharma AD, Singh P, Bhullar SS. Effect of various abiotic stresses on the growth, soluble sugars and water relations of sorghum seedlings grown in light and darkness. BULG. J. Plantphysiol. 2001; 27(1–2):72–84.

Dirik H. Effet du stress hydrique osmotique sur la germination des graines chez les provenances de Cèdre du Liban (Cedrus libani A. Rich.) d’origine Turque. Annals of Forest Science, Springer Verlag/EDP Sciences. 2000;57(4):371-367.