DETERMINATION OF GENETIC POLYMORPHISM OF DN GENES IN WHEAT CULTIVARS RESISTANT TO RUSSIAN WHEAT APHID (Diuraphis noxia)

Main Article Content

UMIDJON BAKHODIROV
SAIDMUROT BABOEV
SODIR MELIEV
FARRUX MATKARIMOV

Abstract

Grain aphids reduce wheat yield by 30 to 75%. As a result of absorption of plant sap by aphids  from the leaves and ears of grain crops, the crops become stunted, and in some cases no ear emergence is observed. Accordingly, this leads to a sharp decrease in the quantity and quality of the yield. In order to determine the genetic polymorphisms of resistance to grain aphids, among 38 samples of local landrace, СIMMYT and local wheat varieties of bread wheat grown in Uzbekistan, 9 SSR primers and PCR amplifications were performed, and this article presents an analysis of these PCR results. Resistance to Russian wheat aphids has been identified in 6 of the Dn genes, namely Dn1, Dn2, Dn4, Dn5, Dn6 and Dn8 genes, and the genotypic polymorphism was studied to be 60%.

Keywords:
Diuraphis noxia, T. aestivum L, wheat, variety, marker, resistance.

Article Details

How to Cite
BAKHODIROV, U., BABOEV, S., MELIEV, S., & MATKARIMOV, F. (2021). DETERMINATION OF GENETIC POLYMORPHISM OF DN GENES IN WHEAT CULTIVARS RESISTANT TO RUSSIAN WHEAT APHID (Diuraphis noxia). PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 22(3-4), 127-133. Retrieved from http://ikpresse.com/index.php/PCBMB/article/view/5866
Section
Original Research Article

References

Dorofeyev VF, Udachin RА, Semenova LV, Novikova МV, Gradchaninova ОD, Shitova IP, Merezhko АF, Filatenko АА. The world wheat. L.VS: Agropromizdat. Leningrad. Dprt. 1987;560.

Mitrofanova ОP. Wheat genetic resources in Russia: status and pre-breeding study. Vavilov Journal of Genetics and Breeding. 2012;16(1):10-20.

Goncharov NP, Kondratenko E.Ya. Wheat origin, domestication and evolution // Bulletin of VSGB. 2008;12(1/2):159-179.

Alekhin VТ. Harmful corn bug and the problem of obtaining high-quality grain. Plant Protection and Quarantine. 2009;(5):6–7.

Bokina IG. Agroecological substantiation of the use of entomophagy in the protection of cereals from aphids in the forest-steppe of Western Siberia: Diss.abst. for DcS on boil. Kinel, 2009;37.

Bokina I.G. Influence of predecessors on the number of cereal aphids and their entomophages in the northern forest-steppe of Driob'e. Plant Protection Bulletin. 2007;2:44–54.

Khujaev Sh.Т., Kholmuratov EА. Integrated protection of plants from pests, as well as the basics of agrotoxicology. Tashkent. 2014;150-152.

Ennahli S, El Bouhssini M, Grando S, Anathakrishnan R, Niide T, Starkus L, Starkey S, Smith CM. Comparison of categories of resistance in wheat and barley genotypes against biotype 2 of the Russian wheat aphid, Diuraphis noxia (Kurdjumov). Arthropod Plant Interactions. 2009;3:45-53.

Du Toit F, Walters MC. Damage assessment and economic threshold values for chemical control of the Russian wheat aphid, Diuraphis noxia (Mordvilko) on winter wheat. In M. C. Walters [ed], Progress in Russian Wheat Aphid (Diuraphis noxia) research in the Republic of South Africa. Technical communication 191. Department of Agriculture, Republic of South Africa. 1984;58-62.

Liu XM, Smith CM, Gill BS, Tolmay V. Microsatellite markers linked to six Russian wheat aphid resistance genes in wheat. Theor Appl Genet. 2001;102:504-510.

Souza E, Bosque-Perez NA, Schotzko DJ, Guttieri MJ, O’Brien K. Registration of three wheat germplasms resistant to Diuraphis noxia. Crop Sci. 2002;42:319-320.

Haley SD, Peairs FB, Walker CB, Rudolph JB, Randolph TL) Occurrence of a new Russian wheat aphid biotype in Colorado. Crop Sci. 2004;44:1589-1592.

Lapitan NLV, Peng JH, Sharma V. A high-density map and PCR markers for Russian wheat aphid resistance gene Dn7 on chromosome 1RS/1BL. Crop Sci. 2007;47:809-818.

Peng J, Wang H, Haley SD, Peairs FB, Laptan NLV. Molecular mapping of the Russian wheat aphid resistance gene Dn2414 in wheat. Crop Sci. 2007;47:2418-2429.

Giordanengo P, Brunissen L, Rusterucci C, Vincent C, van Bel A, Dinant S, et al. Compatible plant-aphid interactions: how aphids manipulate plant responses. Comptes Rendus Biologies. 2010;333(6–7):516–23. Pmid:20541163.

Ananthakrishnan R, Sinha DK, Murugan M, Zhu KY, Chen M-S, Zhu YC, et al. Comparative gut transcriptome analysis reveals differences between virulent and avirulent Russian wheat aphids, Diuraphis noxia. Arthropod-Plant Interactions. 2014;8(2):79–88.

Liu XM, Smith CM, Gill BS, Tolmay V. Microsatellite markers linked to six Russian wheat aphid resistance genes in wheat. Theor Appl Genet. 2001;102:504–510.

Weng Y, Lazar M. Amplified fragment length polymorphism and simple sequence repeat based molecular tagging and mapping of greenbug resistance gene Gb3 in wheat. Plant Breeding. 2002;121(3):218-223.

Mohase Lintle 1, Amie J van der Westhuizen. Glycoproteins from Russian wheat aphid infested wheat induce defence responses. Z Naturforsch C J Biosci. 2002;57(9-10):867-873.
DOI: 10.1515/znc-2002-9-1019.

Ma ZQ, Sorrells ME. Genetic analysis of fertility restoration in wheat using restriction fragment length polymorphisms. Crop Sci. 1995;35:1137–1143.

Özlem Ateş Sönmezoğlu, Begüm Terzi. Comparison of DNA Extraction Protocols for PCR-based Techniques in Wheat. European Journal of Science and Technology. 2009;17:860-865.

Liu BH. Statistical genomics: linkage, mapping and QTL analysis. CRC Press, Boca Raton. Book. 1998;656-611.

Guo X, Elston RC. Linkage informative content of polymorphic genetic markers. Hum Hered. 1999;49:112–118.

Speer MJ. Genetic linkage: concepts and methods. In: Albers MJ (ed) Genetics of cerebrovascular disease. Blackwell, Oxford. 1999;25–26.

Liu XM, Smith CM, Friebe BR, Gill BS. Molecular mapping and allelic relationships of Russian wheat aphid resistance genes. Crop Sci. 2005;45:2273-2280.