INTERACTIVE EFFECT OF PH AND TEMPERATURE ON GROWTH AND BIOCHEMICAL PARAMETERS IN T. aestivum and T. durum VARIETIES

Main Article Content

KRATIKA PATHAK
REKHA GADRE

Abstract

Wheat production is adversely affected by extreme temperature and pH. Moreover, combination of temperature and pH, have a prominent effect on plant growth and development. In the present study seven days old wheat varieties of Triticum aestivum (HI1418, HI1500, HI1531, HI1544, HI1563, HD2932) and Triticum durum (HD4672, HI8381, HI8498, HI8627, HI8663, HI8737) were evaluated for nine combination of different pH (4.0, 5.0, 6.0) and temperature (10°, 20°, 30°C). Seedling were supplemented with one-fourth strength hoag land nutrient solution in static hydroponic condition. Effect of temperature was more prominent in fresh tissue weight, length and chlorophyll concentration. The fresh shoot tissue weight, length and chlorophyll content increased at 20°C and 30°C for all the varieties of T. aestivum and T. durum. While root weight and length marginally affected in both species. Effect of pH was visible for NR activity, protein and proline content. NR activity decreased at pH 6 with increase in temperature whereas at pH 4 and 5 increase in activity was observed up to 20°C in T. aestivum and T. durum varieties. Protein content in shoot tissue of T. aestivum was observed high at pH 4 temperature 10°C while in, root tissue it was pH5 temperature 20°C except HI1544. Whereas in T. durum high protein content was observed at pH 6 temperature 20°C. High proline content was observed at 10°C in both species which decreased with increase in temperature except HI1544, HI1563 and HD2932 at pH4. High protein and low proline content was observed in T. durum as compared T. aestivum. Moreover, at pH 5 low proline and high protein content was observed in HD 4672, HI 8498 and HI 8737 varieties, thus maybe potentially cultivated on non-arable land.

Keywords:
Triticum aestivum, Triticum durum, pH, temperature.

Article Details

How to Cite
PATHAK, K., & GADRE, R. (2021). INTERACTIVE EFFECT OF PH AND TEMPERATURE ON GROWTH AND BIOCHEMICAL PARAMETERS IN T. aestivum and T. durum VARIETIES. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 22(3-4), 41-55. Retrieved from http://ikpresse.com/index.php/PCBMB/article/view/5853
Section
Original Research Article

References

Camargo CEDO, Ferreira Filho AWP, Salomon MV. Temperature and pH of the nutrient solution on wheat primary root growth. Scientia Agricola. 2004;61(3):313-318.

Graham DABDP, Patterson BD. Responses of plants to low, non-freezing temperatures: proteins, metabolism, and acclimation. Annual Review of Plant Physiology. 1982;33(1):347-372.

Vierling E. The roles of heat shock proteins in plants. Annual Review of Plant Biology. 1991;42(1):579-620.

Öncel I, Keleş Y, Üstün AS. Interactive effects of temperature and heavy metal stress on the growth and some biochemical compounds in wheat seedlings. Environmental Pollution. 2000;107(3):315-320.

Panda BC, Nagarajan S, Nagpal KC, Suri DK. X-Ray Diffractometric Technique for Screening High Temperature-Resistant Wheat Varieties. Proceedings of the Indian National Science Academy B. 1979;45:247-254.

Mohebbi S, Mahler RL. The effect of soil pH on wheat and lentils grown on an agriculturally acidified northern Idaho soil under greenhouse conditions. Communications in Soil Science and Plant Analysis. 1989;20(3-4):359-381.

Dalling MJ, Halloran GM, Wilson JH. The relation between nitrate reductase activity and grain nitrogen productivity in wheat. Australian Journal of Agricultural Research. 1975;26(1):1-10.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry. 1951;193(1):265-275.

Bates LS, Waldren RP, Teare ID. Rapid determination of free proline for water- stress studies. Plant and Soil. 1973;39(1):205-207.

Strain HH, Svec WA. Extraction, separation, estimation, and isolation of the chlorophylls. In The Chlorophylls. 19666;21-66.

Srivastava HS. Distribution of nitrate reductase in ageing bean seedlings. Plant and Cell Physiology. 1975;16(6):995-999.

Addae PC, Pearson CJ. Thermal Requirements for Germination and Seedling Growth of Wheat. Australian Journal for Agricultural Research. 1992;43:585-94.

Han Y, Fan S, Zhang Q, Wang Y. Effect of heat stress on the MDA, proline and soluble sugar content in leaf lettuce seedlings. Agricultural Sciences. 2013; 4(05):112.

Figueroa-Bustos V, Palta JA, Chen Y, Siddique KHM. Characterization of root and shoot traits in wheat cultivars with putative differences in root system size. Agromomy. 2018;8:109.

Tóth B, Juhász C, Labuschagne M, Moloi MJ. The influence of soil acidity on the physiological responses of two bread wheat cultivars. Plants. 2020;9:1472.
DOI:10.3390/plants9111472

Muslu A, Ergün N. Effects of copper and chromium and high temperature on growth, proline and protein content in wheat seedlings. Bangladesh Journal of Botany. 2013;42(1):105-112.

Izzo HV, Lincoln MD, Ho CT. Effect of temperature, feed moisture, and pH on protein deamidation in an extruded wheat flour. Journal of Agricultural and Food Chemistry. 1993;41(2):199-202.

Atici Ö, Demir Y, Kocaçalişkan İ. Effects of low temperature on winter wheat and cabbage leaves. Biologia Plantarum. 2003;46(4):603-606.

Pinedo ML, Hernández GF, Conde RD, Tognetti JA. Effect of low temperature on the protein metabolism of wheat leaves. Biologia Plantarum. 2000;43(3):363-367.

Al-Khatib K, Paulsen GM. Photosynthesis and Productivity during High- Temperature Stress of Wheat Genotypes from Major World Regions. Crop science. 1990;30(5): 1127-113.

Kaniuga Z. Chilling response of plants: importance of galactolipase, free fatty acids and free radicals. Plant Biology. 2008;10(2):171-184.

Yaneva IA, Hoffmann GW, Tischner R. Nitrate reductase from winter wheat leaves is activated at low temperature via protein dephosphorylation. Physiologia Plantarum. 2002;114(1):65–72.
DOI:10.1034/j.1399-3054.2002.1140110.x

Townsend LR. Effect of form of N and pH on nitrate reductase activity in lowbush blueberry leaves and roots. Canadian Journal of Plant Science. 1970;50(5):603-605.

Yaneva I, Mäck G, Vunkova-Radeva R, Tischner R. Changes in nitrate reductase activity and the protective effect of molybdenum during cold stress in winter wheat grown on acid soil. Journal of Plant Physiology. 1996;149(1-2):211-216.

Balla K, Karsai I, Bónis P, Kiss T, Berki Z, Horváth Á et al. Heat stress responses in a large set of winter wheat cultivars (Triticum aestivum L.) depend on the timing and duration of stress. PLoS ONE. 2019;14(9): e0222639.
DOI:10.1371/journal.pone.0222639