INVESTIGATION AND IDENTIFICATION OF PHYTOPATHOGENIC AND SAPROPHYTIC Fusarium SPECIES IN THE AGRICULTURAL FIELDS SOIL LAYERS OF THE REPUBLIC OF UZBEKISTAN

Main Article Content

A. G. SHERIMBETOV
SH. E. NAMAZOV
B. SH. ADILOV
D. R. RUZMETOV
KH. R. SADIQOV
S. K. MATYOQUBOV
E. Y. KARIMOV

Abstract

Identification and understanding the distribution pattern of the Fusarium species can help prevent crop diseases and large yield losses.  The objectives of this study were isolation and morphological identification of the main Fusarium pathogens from soil layers of cotton fields in Uzbekistan.  A total of 18 isolates of Fusarium were recovered from soil samples. These isolates were morphologically characterized based on variables as mycelial growth, sporulation, macroconidia length and width, microconidia formation, pigmentation of colonies and formation of chlamydospore, and tested for pathogenicity. 8 isolates were identified as Fusarium oxysporum and 10 as Fusarium solani. Pathogenicity test results indicated that 37,5% (3 out of 8) isolates of Fusarium oxysporum were avirulent and 62,5% (5 out of 8) were virulent. 60% (6 out of 8) isolates of Fusarium oxysporum were avirulent and 62,5% (5 out of 8) were virulent.

Isolates of Fusarium species identified and deposited into the Microbial Culture Collection in the frame of this study can help improve the management control strategies of these soil-borne fungi and create valuable resources for the sustainable use of microbial diversity and its conservation.

Keywords:
Fusarium oxysporum, Fusarium solani, pathogenic, conidia, virulence .

Article Details

How to Cite
SHERIMBETOV, A. G., NAMAZOV, S. E., ADILOV, B. S., RUZMETOV, D. R., SADIQOV, K. R., MATYOQUBOV, S. K., & KARIMOV, E. Y. (2020). INVESTIGATION AND IDENTIFICATION OF PHYTOPATHOGENIC AND SAPROPHYTIC Fusarium SPECIES IN THE AGRICULTURAL FIELDS SOIL LAYERS OF THE REPUBLIC OF UZBEKISTAN. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 21(61-62), 101-108. Retrieved from http://ikpresse.com/index.php/PCBMB/article/view/5647
Section
Review Article

References

Joshi R. A review of Fusarium oxysporum on its plant interaction and industrial use. J Med Plants Stud. 2018;6(3):112–115.

D’Mello J, Placinta CM, Macdonald AM. Fusarium mycotoxins: A review of global implications for animal health, welfare and productivity. Anim. Feed Sci. Technol. 1999;80:183–205.

Perincherry L, Lalak-Kańczugowska J, Stępień Ł. Fusarium-produced mycotoxins in plant-pathogen interactions. Toxins. 2019;11:664.

Jambhulkar PP, Sharma M, Lakshman D, Sharma P. Natural mechanisms of soil suppressiveness against diseases caused by Fusarium, Rhizoctonia, Pythium, and Phytophthora, Organic amendments and soil suppressiveness in plant disease management. Springer. 2015;95-123.

Mandal A, Neenu S. Impact of climate change on soil biodiversity-a review. Agricultural Reviews. 2012;33:283-292.

Campos SB, Lisboa BB, Camargo FO, Bayer C, Sczyrba A, Dirksen P, Albersmeier A, Kalinowski J, Beneduzi A, Costa PB, Passaglia LP, Vargas LK, Wendisch VF. Soil suppressiveness and its relations with the microbial community in a Brazilian subtropical agroecosystem under different management systems. Soil Biol Biochem. 2016;96:191-7.

Mazzola M. Manipulation of rhizosphere bacterial communities to induce suppressive soils J. Nematol. 2007;39:213.

Kwak YS, Weller DM. Take-all of wheat and natural disease suppression: A review. The Plant Pathology Journal. 2013;29:125.

Rahjoo V, Zad J, Javan-Nikkhah M, Mirzaldi Gohari A, Okkhovvat S, Bihamta M. Morphological and molecular identification of Fusarium isolated from maize ears in Iran. Journal of Plant Pathology. 2008;90(3):463–468.

Thomas F. Döring, Dagmar Rosslenbroich, Christian Giese, Miriam Athmann, Christine Watson, Imre Vágó, János Kátai, Magdolna Tállai, Christian Bruns. Disease suppressive soils vary in resilience to stress. Applied Soil Ecology. 2020;149: 103482.

Alabouvette C. Fusarium wilt suppressive soils: An example of disease suppressive soils. Australasian Journal of Plant Pathology. 1999;28:57-64.

Manuel Delgado Baquerizo, Carlos A. Guerra, Concha Cano Diaz, Eleonora Egidi, Jun Tao Wang, Nico Eisenhauer, Brajesh K. Singh, Fernando T. Maestre. The proportion of soil-borne pathogens increases with warming at the global scale 2020, Nature Climate Change. 2020;1-5.

Lafuente R, Maymo-Gatell X, Mas-Castella J, Guerrero R. Influence of environmental factors on plasmid transfer in soil microcosms. Cur. Microbiol. 1996;32:213-220.

Pritchard SG. Soil organisms and global climate change. Plant Pathol. 2011;60(1): 82-99.

Lara Durães Sette, Fernando Carlos Pagnocca, André Rodrigues. Microbial culture collections as pillars for promoting fungal diversity, conservation and exploitation. Fungal Genetics and Biology. 2013;60:2-8.

Nelson PE, Toussoun TA, Marasas WF. Fusarium species: An illustrated manual for identification. Pennsylvania State University: USA. 1983;203.

Leslie JF, Summerell BA. The Fusarium laboratory manual. Oxford, UK: Blackwell Publishing Ltd; 2006.

Kamel -Elsalam KA, Asran AA, Omar MR, Aly AA. Frequency and diversity of Fusarium spp. colonizing roots of Egyptian cottons. Arch. Phytopathol. Plant Protect. 2006;39:165-177.