EFFICIENCY OF SYMBIOTIC NITROGEN FIXATION OF SOY NODULE BACTERIA AFTER PRESERVATION

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ROXILA JURAEVA
JAVLON TASHPULATOV
ABDUVALI IMINOV
XOLMUROD BOZOROV
LYUDMILA ZAYNITDINOVA
SVETLANA KUKANOVA

Abstract

Viability of cells of nodule bacteria Bradyrhizobium japonicum after long term preservation in freeze-dried state and under the layer of mineral oil was studied. It was established that both tested methods of preservation cause no great difference in morphological and cultural features and result in preservation of stability by cells. Field trials revealed that inoculation of seeds by nodule bacteria on standard NPK background greatly enhanced growth, development and formation of nodules, and yield of soy (Orzu variety). The action of initial dose of nitrogen (30, 60, 90) on activity of the nitrogen fixation process and nitrogen nutrition of soy was studied.

 

Keywords:
Nodule bacteria, soy, seeds, inoculation, mineral fertilizers, symbiosis.

Article Details

How to Cite
JURAEVA, R., TASHPULATOV, J., IMINOV, A., BOZOROV, X., ZAYNITDINOVA, L., & KUKANOVA, S. (2020). EFFICIENCY OF SYMBIOTIC NITROGEN FIXATION OF SOY NODULE BACTERIA AFTER PRESERVATION. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 21(61-62), 72-79. Retrieved from http://ikpresse.com/index.php/PCBMB/article/view/5644
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Original Research Article

References

Mavlyanova RF, Zuev VI, Kim VV, Pirnazarov DR. Technology of cultivation of soybeans in Uzbekistan. Tashkent. 2013; 24.

Yormatova DY. Features of technology for cultivation of soybeans in Uzbekistan. Abstract of Thesis. Doctor of Biological Sciences. Tashkent. 1991;34.

Patika VP, Krutilo DV, Kovalevska TM. Infusion of indigenous populations of bulbous soya bacteria on the symbiotic activity of the introduced strain Bradyrhizobium japonicum 634b. J. Microbiology. 2004;66(3):14-21.

Sytnikov DM. Biotechnology of microbial nitrogen fixers and future trends of their preparations application. Biotechnology. 2012;5(4):34-45.

Kavanagh VB, Vessey JK. Symbiotic efficiency of Bradyrhizobium japonicum for development of a commercial soybean inoculants for western Canada. Conference of the Canadian Society of Agronomy and Canadian Society for Horticultural Science. 2006; 86(5):1415.

Dilfuza P. Jabborova, Yuriy I. Enakiev, Kakhramon D. Davranov, Shahjahon A. Begmatov. Effect of co-inoculation with Bradyrhizobium japonicum and Pseudomonas putida on root morph-architecture traits, nodulation and growth of soybean in response to phosphorus supply under hydroponic conditions. J. Bulgarian Journal of Agricultural Science. 2018; 24(6):1004–1011.

Paliya S, Tikle AN, Thomas T. Efficacy of micronutrients in influencing growth behavior of rhizobium of Pigeonpea (Cajanus cajan L. [Millsp.]). Orient. J. Chem. 2014;30:731–736. DOI: 10.13005/ojc/300245

Qin L, Zhao J, Tian J, Chen L, Sun Z, Guo Y, et al. The high-affinity phosphate transporter gmpt5 regulates phosphate transport to nodules and nodulation in soybean. Plant Physiol. 2012;159:1634–1643. DOI: 10.1104/pp.112.199786

Gadimov AG. Starting doses of nitrogen and symbiosis of soybeans with nodule bacteria. Bulletin. 2003;4:44-47.

Agafonov EV, Agafonova LN, Gujvin SA. Application of mineral and bacterial fertilizers for soy. Agrochem. Bulletin. 2005;5:18-20.

Chen J, Li Y, Wen S, Rosanoff A, Yang G, Sun X. Magnesium fertilizer-induced increase of symbiotic microorganisms improves forage growth and quality. J. Agric. Food Chem. 2017; 65:3253–3258. DOI: 10.1021/acs.jafc.6b05764

Borodychev VV, Lytov MN. Soy mineral nutrition. Agrochem. Bulletin. 2005;5:20-22.

Nadkernichnaya EV, Kovalevskaya TM. Influence of free-living nitrogen-fixing bacteria on the formation and functioning of legume-rhizobial symbiosis in some agricultural crops. Physiology and Biochemistry of Cultivated Plants. 2001; 355-362.

Egamberdieva D, Wirth S, Jabborova D, Räsänen LA, Berg G, Liao H. Coordination between Bradyrhizobium and root colonizing Pseudomonas alleviates salt stress in soybean (Glycine max L.) through altering root system architecture and improving nodulation. J. Plant Inter. 2017; 12:100–107. DOI: 10.1080/17429145.2017.1294212

Niu Y, Jin G, Li X, Tang C, Zhang Y, Liang Y, et al. Phosphorus and magnesium interactively modulate the elongation and directional growth of primary roots in Arabidopsis thaliana (L.) Heynh. J. Exp. Bot. 2015; 66:3841–3854. DOI: 10.1093/jxb/erv181

Perry SF. Freeze-drying and cryopreservation of bacte¬ria. Mol. Biotechnol. 1998;9(1):59-64.

Pasarell I, McGinnis MR. Viability of fungal cultures maintained at -70 degress C. J. Clin. Microbiol. 1992;30(4):1000-1004.

Kupletskaya MB, Arkad’eva ZA, Long-Term maintenance of microorganisms in the culture collection of the microbiology Department of the Moscow State University. J. Microbiology. 1997;66/2: 283-288.

Kupletskaya MB, Netrusov AI. Viability of lyophilized microorganisms after 50 years of storage. Microbiology. 2011;80(6):842.

Zolotilina GD, Shakirzyanova MR. Viability of the collection fund of bacteria during long- term storage. Reports of the Uzbek Academy of Sciences. 2005(2):84-90.

Juraeva RN, Bakhtiyorova MS, Kambaralieva MI, Tashpulatov JJ. Vegetable soya – source for isolation of nodule bacteria. J. Microbiology and virology. Almaty. 2015; 70-74.

Methodical recommendations for obtaining new strains of nodule bacteria and assessment of their effectiveness Under. general management of Novikova AT, BV, Simarov L. All-Russian Scientific Research Institute of Agricultural Meteorology. 1979;33.

Guidelines. Methods, techniques for studying and selecting effective strains of soybean nodule bacteria. Analytical selection methods. Blagoveshchensk. 2005; 70.

Methods for agrochemical analysis of soils and plants. Tashkent, ed. Меkhnat. 1977; 49-228.

Islamova Z. The effect of nitragin and mineral fertilizers on soybean yield. J. Agro Ilm. 2011;3(19):15.

Guidelines for the study of samples of the world collection of beans. Edited by Doctor of Biological Sciences. Chekalina NM – L. 1987;28.

Dospekhov BA. Field experiment technique. Moscow; 1985.