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This study was made to explore the tendency of effective permeability of antifungal carbendazim by formulating as o/w nanoemulsion (NE). The NE was made by optimizing the concentrations of oleic acid as oil, Tween 20 as surfactant and acetone as co-surfactant, by unprompted emulsification procedure. They were characterized for transparency, drug content, compatibility, pH, viscosity, stability and size by TEM (transmission electron microscopic) analysis. In vitro antifungal assay were conducted against two phytopathogens, Alternaria alternata and Dreschlera oryzae. The transparent Nano emulsion with narrow size range of oil droplets, appropriate pH and viscosity was obtained. TEM analysis indicated the development of distinct nanosized droplets with size ranging between 80-140 nm. The NEs were stable under variable temperature conditions and have improved permeation through fungal spore cells leading to better results in comparison to the marketed Wettable Powder or suspension of Carbendazim.
El-Aasser MS, Sudol ED. Miniemulsion: Overview of research and application. J Chem Tech Res. 2004;1:21-31.
Anton N, Vandamme TF. Nano-emulsions and Micro-emulsions: Clarifications of the Critical Differences. Pharm Res 2011;28: 978-985.
Janjic JM, Ahrens ET. Fluorine-containing nanoemulsions for MRI cell tracking. Nanomed Nanobiotech. 2009;1:492-501.
Sheikh SS, Shakeel F, Talegaonkar S, Ahmad FJ, Khar RK, Ali M. Development and bioavailability assessment of ramipril nanoemulsion formulation. Eur J Pharm Biopharm. 2007;66(2):227-243.
Shakeel F, Baboota S, Ahuja A, Ali J, Aqil M, Shafiq S. Nanoemulsions as vehicles for transdermal delivery of aceclofenac. AAPS Pharmaceut Sci Tech 2007;89(4):191-99.
Teo BSX, Basri M, Zakaria MRS, Salleh AB, Rahman RNZRA, Rahman MDA. A potential tocopherol acetate loaded palm oil esters-in-water nanoemulsions for nanocosmeceuticals. J Nanobiotech. 2010; 8(1): 4.
Zhang LZ, Wei N, Wu QX, Ping ML. Anti-oxidant response of Cucumis sativus L. to fungicide carbendazim. Pest Biochem Physiol. 2007;89:54–59.
Gea FJ, Tello JC, Navarro MJ. Efficacy and effects on yield of different fungicides for control of wet bubble disease of mushroom caused by the mycoparasite Mycogone perniciosa. Crop Prot 2010;29:1021-1025.
Helweg A. Degradation and adsorption of carbendazim and 2-aminobenzimidazole in soil. Pest Sci. 1977;8:71–78.
Mazellier P, Leroy E, De Laat J, Legube B. Degradation of carbendazim by UV/H2O2 investigated by kinetic modelling. Environ Chem Lett. 2003;1:68–72.
Lin X, Hou Z, Feng Y, Zhao S, Ye J. Isolation and characteristics of efficient carbendazim degradation bacterium. International Conference on Agricultural and Biosystems Engineering Advances in Biomedical Engineering 2011;1–2:384-388.
Ni N, Sanghvi T, Yalkowsky SH. Solubilization and preformulation of carbendazim. Int J Pharm. 2002;244:99-104.
Gumber K, Sidhu A, Kocher DK. Synthesis and preliminary evaluation of carbendazim nanoemulsions as larvicidal agent against Culex mosquitoes. Adv Appl Res 2017; 9(1):7-11.
Jain A, Deveda P, Vyas N, Chauhan J, Khambete H, Jain S. Development of antifungal emulsion based gel for topical fungal infection(s). Int J Pharm Res Dev. 2011;12:18-25.
Leng P, Zhang ZH, Li Q, Zhang Y, Zhao M Development of a difenoconazole/ propiconazole microemulsion and its antifungal activities against Rhizoctonia solani AG1-IA. Die Pharmazie. 2012;67: 534–541.
Leng P, Liu L, Li Q, Zhao M, Pan G. Preparation of triadimefon microemulsion and its antifungal activity against Rhizoctonia solani AG1-IA Analysis. Curr Pharm Anal. 2013;9:396-403.
Devi TR, Chhetry GKN. Evaluation of antifungal activities of certain plant against Fusarium udum Butler causing wilt in pigeonpea (Cajanus cajan(L.) Millsp.). Int J Sci Res Pub. 2012;2:1-4.
Modi JD, Patel JK. Nanoemulsion-Based Gel Formulation of aceclofenac for topical delivery. Int J Pharm Pharmaceut Sci Res. 2011;1:6-12.