ANTIBACTERIAL EFFICACY OF SOME STANDARD ANTIBIOTICS DISCS COATED WITH BIOLOGICALLY SYNTHESIZED SILVER NANOPARTICLES FROM Cassia occidentalis LEAVES

Main Article Content

LAWAL DANJUMA
UMAR ABDULLAHI

Abstract

Biologically produced silver nanoparticles are being used extensively in the area of medicine. Extracellular biosynthesis of silver nanoparticles was carried out by using Cassia occidentalis leaves extracts for the reduction of aqueous silver ions in short phase. This research is aimed at determining the antibacterial efficacy of some standard antibiotics discs coated with biologically synthesized silver nanoparticles from Cassia occidentalis leaves. Preliminary phytochemical analysis of aqueous extracts revealed the presence tannins, flavonoids, saponins, glycosides and triterpenoids. The silver nanoparticles formation was confirmed by the colour change of plant extracts from yellow to dark brown. Standard antibiotics discs were coated with Phytosynthesized silver nanoparticles at various concentrations and tested for antibacterial activity using disc diffusion method. The test cultures include S. typhi, P. aeruginosa, K. pneumoniae and E. coli. The antibacterial activity of standard antibiotics discs coated with Phytosynthesized silver nanoparticles was analyzed by measuring the zone of inhibition. The result indicated that S. typhi, K. pneumoniae, P. aeruginosa and E. coli are sensitive; similarly the MIC result shows that S. typhi and E. coli are sensitive at 250µg/ml concentration whereas K. pneumoniae and P.aeruginosa are sensitive at 500 µg/ml; the MBC result indicated that E. coli, S. typhi and K. pneumoniae are sensitive at 250 µg/ml, 500 µg/ml, 1000 µg/ml respectively while P. aeruginosa was resistant. Antibiotics discs coated with phytosynthesized AgNPs showed higher antibacterial effects against the bacterial isolates when compared with standard antibiotics alone.

Keywords:
Standard-antibiotics, biosynthesis, AgNPs, Cassia occidentalis, bioassay, MIC, MBC.

Article Details

How to Cite
DANJUMA, L., & ABDULLAHI, U. (2021). ANTIBACTERIAL EFFICACY OF SOME STANDARD ANTIBIOTICS DISCS COATED WITH BIOLOGICALLY SYNTHESIZED SILVER NANOPARTICLES FROM Cassia occidentalis LEAVES. Journal of Medicine and Health Research, 5(1), 47-53. Retrieved from http://ikpresse.com/index.php/JOMAHR/article/view/5845
Section
Original Research Article

References

Prabhu N, Divya TR, Yamuna G. Synthesis of silver phyto nanoparticles and their antibacterial efficacy. Digest. J. Nanomater. Biostruct. 2010;5:185-189.

Murray CK. Infections in burns. J. Trauma. 2007;62:S73.

Hancock RE. The end of an era? Nat. Rev. Drug Discov. 2007;6:28.

MacGowan JEJ. Year 2000 bugs: the end of the antibiotic era. Proc. R. Coll. Physicians Edinb. 2001;31:17–27.

Dai, T. Topical antibacterials for burn wound infections. Recent Pat. Antiinfect. Drug Discov. 2010;5:124–151.

El Kenawy R, Worley SD, Broughton R. The chemistry and applications of antibacterial polymers: a state-of-the-art review. Biomacromolecules. 2007;8:1359–1384.

Hetrick EM, Schoenfisch MH. Reducing implant-related infections: active release strategies. Chem. Soc. Rev. 2006;35:780–789.

Jeong SH, Yeo SY, Yi SC. The effect of filler particle size on the antibacterial properties of compounded polymer/ silver fibers. J. Mat. Sci, 2005;40:5407-5411.

Krutyakov YA, Kudrynskiy A, Olenin AY, Lisichkin GV. Extracellur biosynthesis and antibacterial activity of silver nanoparticles. Russ. Chem. Rev. 2008;77:233.

Sharma VK, Yngard RA, Lin Y. Silver nanoparticles: Green synthesis and antibacterial activities. Adv. Coll. Int. Sci. 2009;145:83-96.

Sharma VK, Yngard RA, Lin Y. Silver nanoparticles: Green synthesis and antibacterial activities. Adv. Coll. Int. Sci. 2009;145:83-96.

Ip M, Lui SL, Poon VKM, Lung I, Burd A. Antibacterial activities of silver dressings: An in vitro comparison. J. Medical. Microbial. 2006;55:59-63.

Bhyan SB, Alam MM, Ali MS. Effect of plant extracts on Okra mosaic virus incidence and yield related parameters of Okra. Asian. J. Agric. Res. 2007;1:112-118.

Calvo MA, Angulo E, Costa-Batllori P, Shiva C, Adelantado C, Vicente A. Natural plant extracts and organic acids: Synergism and implication on piglet’s intestinal microbiota. Biotechnology. 2006;5:137-142.

Saxena A, Tripathi RM, Singh RP. Biological Synthesis of silver nanoparticles by using Onion (Allium cepa) extract and their antibacterial activity. Digest. J. Nanomater. Biostruct. 2010;5:427-432.

Khandelwal N, Singh A, Jain D, Upadhyay MK, Verma HN. Green synthesis of silver nanoparticles using Argimone mexicana leaf extract and Evaluation of their antibacterial activities. Digest. J. Nanomater. Biostruct. 2010;5:483-489.

Thirumurgan A, Tomy NA, Jai GR, Gobikrishnan S. Biological reduction of silver nanoparticles using plant leaf extracts and its effect an increased antibacterial activity against clinically isolated organism. De. Phar. Chem. 2010;2:279-284.

Thirumurguan G, Shaheedha SM, Dhanaraju MD. In vitro evaluation of antibacterial activity of silver nanoparticles synthesized by using phytothora infestans. Int. J. Chem Tech Res. 2009;1:714-716.

Blumgarten AS. Senna. In: blumgarten, A. S. (Ed.) Textbook of Material, Medical, pharmacology and Therapeutics, 7th Edition, John Wily and Sons. New York. 1937;225– 226.

Mann A, Gbate M, Umar AN. Senna occidentalis (Linn.). Medicinal and economic plants of Nupe land. Jube Evan Books and Publication, Kaduna. 2003;41.

Jain SC, Sharma RA, Jain R, Mittal C. Antibacterial screening of Cassia occidentalis L in vivo and in vitro. Phytotherapy Research. 1998;12:200-204.

Saganuwan AS, Gulumbe ML. Evaluation of in vitro antibacterial activities and phytochemical constituents of Cassia occidentalis. Animal Research International. 2006;3:566-569. Tona et al., 1999

Tona L, Cimanga RK, Mesia K, Musuamba CT, De Bruyne T, Apers S, Hernans N, Miert SV, Pieters L, Totté J, Vlietinck AJ. In vitro antiplasmodial activity of extracts and fractions from seven medicinal plants used in the Democratic Republic of Congo. Journal of Ethnopharmacology. 2004;93:27-32.

Sharma N, Trikha P, Athar M, Raisuddin S. In vitro inhibition of carcinogen induced mutagenicity by Cassia occidentalis and Emblica officinalis. Drug and Chemical Toxicology. 2000;23:477-84.

Yadav JP, Arya V, Yadav S, Panghal M, Kumar S, Dhankhar S. Cassia occidentalis: A review on its ethnobotany, phytochemical and pharmacological process. Fitoterapia; 2009. DOI: 10.1016/j.fitote.2009.09.008.

Harbone JB. Phytochemical methods. A guide to modern techniques of plant analysis. 3rd Edn. Chapman and Hall Int. Edn, New York; 1998.

Kokate CK. Pharmacognosy 16th Edn. Nirali Prakashan, Mumbai, India. 2001.

Lawal D, Mukhtar MD, Taura DW, Bukar A. Phytochemical screening and in vitro anti-bacterial studies of the ethanolic extract of A. comosus (Linn) Merr. Peels on Aeromonas hydrophila and Salmonella species. Bayero Journal of Pure and Applied Sciences. 2011; 4(1):168-172.

Odebiyi EO, Ramstard AH. Investigation phytochemical screening and antibacterial screening of extracts of Tetracarpidium conophorum. Journal of Chemical Society of Nigeria. 1978;26:1.

Waterman PG. Methods in plant biochemistry. Academic Press. 1993; 2. Vol. 8.

Savithramma N, Linga RM, Rukmini K, Suvarnalatha DP. Antimicrobial activity of silver nanoparticles synthesized by using medicinal plants. International Journal of ChemTech Research. 2011;3(3).

NCCLS. Performance standards for antibacterial disk susceptibility tests: Approval standard M2-A7 7th edition. Pennsylvania: Clinical and Laboratory Standards Institute. 2000.

Lawal, D, Isa Y, Idris B. Phytochemical analysis and synergistic action of Annona comosus (Linn) Merr. peel and Citrus senensis peel extracts on Aeromonas hydrophila and Salmonella species. Bayero Journal of Pure and Applied Sciences. 2013;6(1):40-45.

Anwar F, Latif S, Ashraf M, Gilani AH. Moringa oleifera: A food plant with multiple medicinal uses. Phytotherapy Research. 2007; 21(1):17-25.

Savithramma N, Linga RM, Ankanna S. Screening of medicinal plants for secondary metabolites. Middle East Journal of Scientific Research. 2011;8(3).

Mohamed M, Raja M, Ahmed JS. Antibacterial potential of silver nanoparticle synthesized by marine actinomycetes in reference with standard antibiotics against hospital acquired infectious pathogens. African Journal of Biotechnology. 2016;15(38):2115-2123.

Mostafa MH, Khalil EH, Ismail K Z, El-Baghdady DM. Green synthesis of silver nanoparticles using Olive leaf extract and its antibacterial activity. Arabian Journal of Chemistry. 2014;7:1131-1139.

Ahmad A, Mukherjee P, Senapati S, Mandal D, Khan MI, Kumar R Sastry M. Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum. Colloids Surf. B Biointerfaces. 2016;28:313-318.

Amanulla MF, Kulandaivelu B, Morukattu G, Ruchi Y, Pudupalayam TK, Ramasamy V. Biogenic synthesis of silver nanoparticles and their synergistic effect with antibiotics. Nanomedicine: Nanotechnology, Biology and Medicine. 2010;6:103-109.

Martinez GA, Nino-martinez N, Martinez-Gutierrez F, Martinez-Mendoza JR, Facundo Ruiz. Synthesis and antibacterial activity of silver nanoparticles with different size. J Nanopart Rsc. 2008;10:1343-1348.

Awwad AM, Salem NM, Abdeen AO. Green synthesis of silver nanoparticles using carob leaf extract and its antibacterial activity. International Journal of Industrial Chemistry. 2013;4:29.